Skip Ribbon Commands
Skip to main content

Research Facilities

openlabconcept.png

​​Research Platforms 

Our research platforms cover imaging, flow cytometry, histology and other essential services to better support research at LKCMedicine. Where possible, new cutting-edge technologies or equipment are centrally acquired and shared.


Core Platforms

Advanced Optical Bio-Imaging Platform

The imaging platform has microscopes that satisfy the various imaging needs of laboratories in Clinical Sciences Building (CSB), Experimental Medicine Building (EMB) and Academia (SGH).

The imaging platform comprises upright and inverted wide-field fluorescence microscopes, calcium imaging system, slide scanner, high-end laser scanning confocal microscopes, lightsheet microscope, high-content screening (HCS) microscopes, TIRF/N-STORM super-resolution microscope, two-photon microscopes and mesoscope.


Stem Cell and Flow Cytometry Platform

The stem cell facility is a core facility that provides a platform for animal and human pluripotent stem cell research. The facility facilitates the growth and conduction of experiments using embryonic stem cells and induced pluripotent cells.

The Flow Cytometry Core Facility provides researchers with cell-sorting services and access to a Fortessa Analyzer.


Histology Suite

The knowledge of normal histologic appearances is essential.

It allows one to recognise abnormal or diseased structures and to comprehend how biochemical and physiological processes cause disease and allowing the diagnosis and research of certain diseases like cancer and diabetes.

Thus, histology serves a crucial component in the science of pathology. Establishing a histology facility at LKCMedicine is essential to ensure that this anatomy-based approach is always made available to all research and teaching faculties.


Genomics Platform

The next stage of genomic research is the derivation of meaningful knowledge from the DNA sequence.

Research studies that build on the work of Genome Projects are under way worldwide.

The Genomics platform at LKCMedicine is a shared resource offering DNA extraction, DNA library preparation, next-generation sequencing (NGS) and DNA expression technologies that revolutionised genomics research.

Radiation Research Platform

The use of radioactive trace substances and ionising radiation is common in biomedical research.

The basic work is normally carried out in the laboratory and using animal models to study chemical, physiological and metabolic processes, as well as the turnover of pharmaceuticals.

The Radiation Research Platform at LKCMedicine is a shared resource platform offering radio-ligands production, purification, and radioactivity detection technologies.


Glassware Washing Facility

Centralised glassware washing facilities, at level 3 of CSB and level 2 of EMB, are each fully equipped with two sterilisers and two glassware washers to serve the teaching and research laboratories of the School.

Researchers at both campuses can utilise the facility to wash and autoclave their glassware, as well as sterilise their media and biohazard materials.

The glassware is routinely collected, washed and stored at designated cabinets by dedicated facility staff.


Fee-for-Service Platforms

Non-Alcoholic Fatty Liver Disease Investigation Centre (NICE)

The Non-Alcoholic Fatty Liver Disease Investigation Centre (NICE) offers the novel LIDPAD model as fee-for-service, including pharmacological and nutritional intervention studies. In the LIDPAD model, non-alcoholic steatohepatitis (NASH) develops within a few weeks and offers an opportunistic window for pharmacological interventions by drug candidates. This model will be beneficial for mechanistic and intervention studies on fatty liver, fibrosis, cirrhosis, and diabetes, among others. 


Lipidomics Platform


LKCMedicine has established a triple quadrupole mass spectrometer with LC which allows quantitation of metabolites of interest for targeted lipidomics studies. The platform enables us to know the chemistry of the metabolites, as well as offers the advantage of quantitative analysis, which is important for (i) confirming results from non-targeted screens, (ii) metabolic modelling and predictions, and (iii) diagnostics.

For non-targeted approach, an additional high resolution Time-of Flight mass spectrometer with upfront separation tools is available. Spectra can be obtained and computation approaches can be developed for comparative lipidomics to determine differences in ‘lipid profiles’. This approach is semi-quantitative but offers the advantage of discovering novel metabolites which are not known previously.

Ion Channel Screening Platform

Ion channels are involved in diverse physiological processes and their malfunction causes numerous human diseases.

Drugs that modulate ion channels account for the second largest group in the pharmacopeia. Methods to study ion channels include ligand-binding, flux-based and fluoroscence-based assays, but the best technology to study ion channel function and screen for compound interaction is the patch clamp technique. This technique is an FDA-mandated hERG (Human Ether-a-gogo-related Gene) safety testing.


Zebrafish Facility
zebrafishfac.png
Zebrafish are a major model organism for biomedical research, due to relatively low costs, and ease of imaging and genetic manipulation. The Zebrafish Facility houses 2760 tanks and will provide researchers from Singapore with access to a range of wild-type, transgenic and mutant zebrafish lines. The facility also contains a quarantine room and can assist with the import and export of embryos, husbandry and IVF procedures, and has a nursery with robotic feeder for optimal growth rates. Procedure rooms are equipped with fluorescent microscopes for imaging as well as injection apparatus for generation of transgenics or knockouts.

Rental

The research core facilities encompass a broad range of services, technology and expertise. The multidisciplinary scope of core facilities encourages sharing of scientific resources to enable focused development of technology that serves all research efforts across the spectrum of biomedical science. As a guiding principle, all faculty, their staff and students may utilise these shared resources.

Below is the list of approved charging rates for core facilities equipment and specialised equipment. The charging rates are effective from January 2018.

To download the Research Platforms brochure, click here​​. 

For any enquiries, please contact Ng Sean Pin @ seanngsp@ntu.edu.sg and Ken Wong @ kenwongkn@ntu.edu.sg.

​​​Advanced Microscopy Facility ​Location ​​​User fee ($/hour)​
​​ ​​ NTU Others
Slide Scanner  CSB, Level 11 20 ​30​
Inverted Fluorescence Live Cell Microscope - DMI6000 CSB, Level 17 ​20 ​30​
Inverted Fluorescence Live Cell Microscope - AO7 ​CSB, Level 11  20​ 30​
​Upright Confocal Microscope - SP8 CSB, Level 17
50​ 60​
​Upright Confocal Microscope - SPE CSB, Level 17 15 20​
Inverted Confocal Airyscan Microscope with live cell imaging capability - LSM800 ​CSB, Level 11 50 60
Upright Confocal Microscope - DM5500 EMB, 03-02Y
15 20
Upright Confocal Microscope - TiE EMB, 03-02Y 15​ 20​
Inverted Confocal Microscope with Airyscan - LSM800​ ​EMB, Level 7 50 60
Upright Confocal Microscope - LSM800 ​EMB, Level 4 35​ 45​
Inverted Fluorescence Live Cell Microscope in a closed box - CD7 ​EMB, Level 7 20​ 30​
High Content Screening Microscope - Operetta CLS CSB, Level 11​ 45​ 60​
High Content Screening Microscope - IN Cell 2200 EMB, Level 7 ​ 45​ 60​
​​Lightsheet Microscope EMB, Level 4​ 60​ 75​

Flow Cytometry Facility ​​Location ​​​User Fee ($/Hour)​
​​ ​​ NTU ​Others
​BD FacsMelody (3-lasers)
(User Operated)
​EMB, Level 7 85​ ​115
​BD FacsMelody (3-lasers)
(Staff Operated)
​EMB, Level 7 ​120 150​
​BD FacsAria Fusion (4-lasers)
(Staff Operated Only)
​CSB, Level 11 ​125 175​
​BD Fortessa X-20 Analyser (5-lasers)
(User Operated)
​EMB, Level 7
CSB, Level 11
​40 60​
BD LSRFortessa X-20 Analyser (5-lasers)
(Staff Operated)
EMB, Level 7
CSB, Level 11
75 ​95​

Specialised Equipment ​Location User Fee ($/Hour)​
​​​​​
​​ ​​ NTU ​Others
Perkin Elmer TopCount Scintillation Counter EMB, Level 7 20 ​30​
​​
Glassware Washing Facility Location ​​​User Fee ($/Hour)​ ​ ​
​​ ​​ NTU Others
Glassware washing service per basket E​MB, Level 2
CSB, Level 3
20 30

Core Research Platform @ NTU - Histology Facility ​Location ​​​​User Fee ($/Hour)​ ​ ​
​​ ​​ NTU ​Others
Leica Automated Tissue Processor ASP6025
E​MB, Level 7 20 30
​Leica HistoCore Pearl Tissue Processor CSB, Level 12​ 20​ 30​
​Leica Crytostat CM3050S ​CSB, Level 11 & 12 
E​MB, Level 7
​10 15
Leica Microtome RM2255 ​CSB, Level 11 & 12
EMB, Level 7
10​ 15​
Leica Tissue Embedder EG1150  ​CSB, Level 11 & 12
EMB, Level 7
10​ 15
​Leica Autostainer XL ​CSB, Level 12 &
EMB, Level 7
20​ 30
​​
​​Lipidomics Platform Location​ user Fee ($/Hour)​ ​
NTU Others​
SCIEX Triple Quad 6500 + LC-MS/MS System ​(Targeted) EMB, Level 5​ 250 per sample​ 350 per sample​
​SCIEX TripleTOF 6600 + System (Untargeted) EMB, Level 5  ​400 per sample 600 per sample
 


Not sure which programme to go for? Use our programme finder
Loading header/footer ...