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Stem Cell Core Platform

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The Stem Cell Core Platform functions as a multi-user shared research facility dedicated to support and accelerate studies involving human stem cells at LKCMedicine and in the industry. 

The Platform
helps maintain and provide well-characterised human embryonic stem cell (hESC) lines and human-induced pluripotent stem cell (iPSC) lines. Training is also provided in essential culture techniques for human pluripotent stem cells. 

The facilities consist of:  

  • Automated cell counters
  • Real-time cell analysers
  • Cell storage freezers and tanks
  • Fully equipped tissue culture facilities with BSCs, CO2 incubators, waterbath, centrifuges, fridges, microscopes, etc.​
 
​Services include:

  • Methods, with further characterisation
  • Two-day hands-on class on the basic methods of culturing human embryonic and pluripotent stem cells (hESC and HiPSC) 
  • Distribution of hESC and hiPSC from LKCMedicine stem-cell bank
  • Use of the facility to culture and expand customised hESC and iPSCs
  • Consultation and support for internal and external stem cell projects
  • Gene-targeting in iPSCs using the CRISPR/Cas9 to generate single gene knockouts

For further enquiries, please contact Dr Ng Sean Pin at seanngsp@ntu.edu.sg or Mr Ken Wong at kenwongkn@ntu.edu.sg.

Equipment

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BIORAD GENE PULSER EXCELL (Custom).png
LONZA 4D- Nucleofactor
Core
, X & Y unit
Biorad Gene Pulser
Excell Total Sytem


 

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