SPC: LC-MS & MS Imaging Technologies

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LC-MS has become a widespread technology within biological and clinical research, with tremendous growth being seen in recent years. It achieves analytical specificity superior to that of immunoassays or conventional high performance/pressure liquid chromatography (HPLC) for low molecular weight analytes.

Omics phenotyping characterizes important biochemical changes caused by different conditions. These include the onset and course of clinical diseases, genetic modifications and the effects of therapeutic interventions, nutrition and life-style. This phenotyping approach has a large bearing on drug discovery and drug development. In addition, in the quest to achieve a true systems biology understanding of organisms and their diseases, it plays a significant role in integrating knowledge at all levels of biology.

Our LC-MS facilities at SPC include high resolution quadrupole/time-of-flight (QToF) mass spectrometers for untargeted profiling and biomarker discovery, and tandem quadrupoles (TQ) for quantitative targeted analysis of biomolecules. We also have nanoLC with ion mobility ToF MS systems for challenging proteomics measurements consisting of complex mixtures of proteins covering several orders of magnitude dynamic range and where sample quantity is limited. Ion mobility seperation allows biomolecules to be seperated not only by thir hydrophobic/hydrophilic properties (RT) and mass (m/z), but also by their size and shape (collisional cross section). Being the first of its kind in Singapore, we also have MALDI-MS imaging ​capability and DESI-MS imaging ​capability for metabolite localisation and distribution studies on tissue samples. Ion mobility separation allows biomolecules to be separated not only by their hydrophobic/hydrophilic properties (RT) and mass (m/z), but also by their size and shape (collisional cross-sections).

 

QTOF (Custom).jpgWaters UPLC/ Xevo G2-S QTof for exploratory analysis

Xevo® G2-S QToF is designed for scientists who need to identify, quantify, and confirm the broadest range of compounds in the most complex and challenging samples.

Incorporating StepWave™ ion optics for unsurpassed levels of durable sensitivity, the Xevo G2-S QTof also uses proven quantitative time-of-flight (QuanTof™) technology to deliver superior UPLC®-compatible mass resolution*, matrix-tolerant dynamic range**, quantitive performance, mass accuracy and speed of analysis – simultaneously.

Integration with UltraPerformance LC® delivers the highest quality, most comprehensive information, and enables you to make the right decisions, quickly and confidently.*

 

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Waters UPLC/ Xevo Tandem Quadrupole for targeted analysis
 

 

Thanks to a revolutionary off-axis ion source technology known as StepWave™ Xevo® TQ-S delivers unprecedented levels of sensitivity, selectivity, and accuracy.

All Xevo TQ system are designed for quantitative UPLC®/MS/MS application. With the increased sensitivity of the Xevo TQ-S, you can quantify and confirm trace components at even lower levels in the most complex samples.

Best of all, Xevo system allows you to achieve your goals with unparalleled speed and ease.

With Xevo TQ-S, suddenly your laboratory will develop methods to advance biological and medical research, bring drugs to market faster, identify a broader array of food or environmental contaminants, or report reliable forensic data with total confidence.*

 

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Waters UPLC/ SYNAPT HDMS MALDI for proteomics and imaging

 

The SYNAPT G2-Si System provides a unique platform to further our discovery efforts by providing capabilities that go beyond conventional MS instrumentation.

SYNAPT High Definition Mass Spectroscopy® is the combination of high-efficiency T-Wave ion mobility measurements and separations with high-performance tandem MS, enabling the differentiation of samples by size, shape and charge, as well as mass.

By introducing the orthogonal dimension of gas-phase ion mobility separation, you can take advantage of a molecule’s collision cross section to significantly enhance separation specificity, sensitivity and structural insight in your analysis​.*​

 

*Contents adopted from Waters Corporation​​​​​​​​​​